Vinasse is an aqueous effluent of the distillation unit in the sugar-alcohol industry and a problem to the sector due to its potential effects as an environmental pollutant. However, proper usage of vinasse contributes to improving soil quality and agricultural productivity. The objectives of this study were to evaluate the effects of sugarcane vinasse on soil chemical properties and initial growth attributes of the sugarcane plant. The research consisted of a laboratory and a pot experiment. In the laboratory experiment, concentrated vinasse (volume 3 1:10) was applied to soil at the level of 40 m /ha, and non-concentrated vinasse was applied to soil in four levels; viz. 40, 60, 80, and 120 m3/ha to evaluate soil chemical properties (pH, electrical conductivity, organic matter, nitrogen. phosphorus, and potassium). Data were collected up to for 98 days in the laboratory experiment. Similar treatments were applied for soil pot culture grown varieties, SL 83 06 and SL 96 128 as the above ratios of concentrated and non-concentrated vinasse under net house condition. The results of the laboratory experiment indicated that the concentrated vinasse-treated soil samples showed considerably higher values for all tested chemical properties except soil pH. Both varieties had performed 3 well in 40 m /ha non concentrated vinasse level. However, the variety, SL 83 06 showed higher performance than SL 96 128 in shoot dry weight, root length, shoot dry weight, and 3 root dry weight at 40 m /ha non-concentrated vinasse level. Since, findings indicated that, lower doses of non-concentrated vinasse are more favorable to plant growth with a stimulatory effect on plant initial growth.